Optimization of Biofloc Technology for Intensive Pacific White Shrimp (Litopenaeus vannamei) Culture: Effects on Water Quality, Growth Performance and Microbial Community Structure

Authors

  • Minh Nguyen

    Research Institute for Aquaculture No. 2 (RIA2), Ho Chi Minh City 700000, Vietnam

Keywords:

Biofloc technology; Litopenaeus vannamei; Water quality regulation; Growth performance; Microbial community

Abstract

This study optimized biofloc technology (BFT) parameters for intensive Litopenaeus vannamei culture. Three carbon sources (molasses, wheat bran, glucose) and two C/N ratios (15:1, 20:1) were tested in 90-day indoor trials. Water quality indicators, shrimp growth performance and microbial communities were monitored. Results showed molasses at C/N 20:1 significantly reduced ammonia-N (32.6±1.8 mg/L) and nitrite-N (2.1±0.3 mg/L) (P<0.05), increased weight gain rate (286.4±12.7%) and survival rate (89.2±3.1%). 16S rRNA sequencing revealed dominant genera including Vibrio (12.3%) and Pseudomonas (8.7%). This optimization provides theoretical support for sustainable intensive shrimp culture.

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