Varietal purity evaluation in sesame (Sesamum indicum L.) seeds by means of random amplified polymorphic DNA (RAPD) markers in Venezuela
DOI:
https://doi.org/10.55121/nc.v2i2.39Abstract
BACKGROUND: Sesame is an important crop due to the excellent quality oil derived from its grains. With as any other crop, the seed used as initial input in the agricultural process, is the first warranty of success that a farmer has. The quality seed must be evaluated during the multiplication process, to be sure that varietal purity, as component of genetic quality, is not affected. OBJECTIVES: The general objective was to determine ability of random amplified polymorphic DNA (RAPD) markers to evaluate varietal purity; two specific objectives were defined: to evaluate the ability of RAPD to detect seeds of non-target cultivars (seed contaminants), and to evaluate the ability of RAPD to detect troubles during seed multiplication. MATERIAL AND METHODS: For first objective seeds of cultivar UCLA-1 were mixed with seeds of another known accession, in different proportions: 99:1, 98:2, 97:3 and 95:5. RAPD markers were performed on these different proportions, and band pattern of each proportion was compared to UCLA-1 and to the other accessions band pattern. For second objective, RAPD was performed on samples from genetic seed, and seeds of the two following multiplication cycles. RESULTS: RAPD was able to identify seed contaminant in proportion as low as 1%. For second objective, RAPD identified the three samples from genetic seed with the same band pattern, but it was different to band pattern of the other two kinds of seed. Therefore, RAPD had the ability to identify that the multiplication of seeds was not totally suitable, because some non-target seeds appeared as source of contamination. CONCLUSION: RAPD proved to be an effective tool to monitor genetic quality, specifically varietal purity, in sesame seed.
Keywords:
seed quality, genetic quality, molecular marker, DNA, random amplified polymorphic DNA, polymorphismReferences
[1] Bedigian D, Harlan J: Evidence for cultivation on sesame in the ancient world. Economic Botany. 1986, 40: 137-154. http://dx.doi.org/10.1007/BF02859136
[2] Ashri A: Sesame breeding. Plant Breeding Reviews. 1998, 16: 179-228. https://doi.org/10.1002/9780470650110.ch5
[3] FAO, 2022. FAOstat Databases. [http://faostat.fao.org/]
[4] Laurentin, H. 2020. Introducción. In: Producción de Semilla en Venezuela (Ed. Hernán Laurentin). Ediciones Astro Data S.A. Sociedad Venezolana de Mejoramiento Genético y Biotecnología Agrícola. Maracaibo, Venezuela
[5] Laurentin, H. 2011. Genética Agrícola. Editorial Académica Española. Saarbrücken, Germany.
[6] Montilla, D. and Teran, H. 1996. UCLA1, Una Nueva Variedad de Ajonjolí, Bioagro. 8, 26-29
[7] Laurentin, H., Montilla, D., and V. Garcia. Interpreting genotypexenvironment interaction in sesame (Sesamum indicum L.), The Journal of Agricultural Sciences 145, 263-271 https://doi.org/10.1017/S0021859606006654
[8] Clarke, B., L. Moran and R. Appels, 1989. DNA Analysis in Wheat Breeding, Genome. 32, 334 – 339. https://doi.org/10.1139/g89-450
[9] Rohlf F: NTSYS-pc:numerical taxonomy and multivariate analysis system, version 2.11T. 2004, New York, Exeter Software
[10] Ramos, N., K. Brunelli, L. Aranha, J. Filho, 2006. Sensibilidade dos microssatélites para determinar pureza varietal em sementes se milho. Revista Brasileira de Sementes, 28(1), 99-105. https://doi.org/10.1590/S0101-31222006000100014
[11] Vasile, V, M. Ciuca, C. Voaides, C. Cornea. 2020. DNA-based methods used for varietal purity detection in wheat cultivars, AgroLife Scientific Journal 9(1), 342-354
[12] Ilbi, H. 2003, RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum. Scientica Horticulturae 97(3-4), 211-218 https://doi.org/10.1016/S0304-4238(02)00158-9
[13] Prashat, D. and V. Ponnuswami, 2008. Determination of F1 hybrid purity of Capsicum annum L. using RAPD markers. Vegetable Sciences 35(2), 190-191
[14] Rana, M., S. Singh and K. Bhat. 2007. RAPD, STMS and ISSR markers for genetic diversity and hybrid seed purity testing in cotton. Seed Science and Technology, 35(3), 709-721 https://doi.org/10.15258/sst.2007.35.3.17
[15] Singh, N., M. Singh, S. Kumar, R. Kumar, V. Singh, H. Prasanna and M. Rai. 2007. RAPD markers for hybrid seed purity testing in tomato (Solanum lycopersicum L.), Current Science. 93, 462-463.
[16] Mylonas, I., A. Georgiadis, A. Apostolidis, K. Bladenopoulos and M Koutsika-Sotiriou. 2014. Barley cultivar discrimination and hybrid purity control using RAPD markers, Romanian Biotechnological Letters. 19(3), 9421-9428.
[17] Virk, P., H. Newbury, M. Jackson and B. Ford-Lloyd, 1995. The identification of duplicate accessions within a rice germplasm collection using RAPD analysis, Theoretical and Applied Genetics. 90, 1049-1055. https://doi.org/10.1007/BF00222920
[18] Simon, A., V. Subbiah, C. Tyng and N. Yusuf, 2020. Genetic diversity of Sabah rice cultivars using random amplified polymorphic DNA (RAPD) markers. Borneo International Journal of Biotechnology (BIJB). 1, 35-43. https://doi.org/10.51200/bijb.vi.1991
[19] Perveen, R., S. Jahangir and S. Hassan, 2020. Genetic diversity in selected maize genotypes of Pakistan by using rapid amplified polymorphic DNA technique, University of Wah Journal of Science and Tehnology (UWJST). 4, 15-19. https://www.uwjst.org.pk/index.php/uwjst/article/view/51
[20] Nazarzadeh, Z., H. Onsori and S. Akrami, Genetic diversity of bread wheat (Triticum aestivum L.) genotypes using RAPD and ISSR molecular markers, Journal of Genetic Resources. 6(1), 69-76. doi 10.22080/jgr.2020.18262.1172
Downloads
How to Cite
Issue
Section
License
Copyright (c) 2023 Edickson Frances, Hernán Laurentin
This work is licensed under a Creative Commons Attribution 4.0 International License.
Submit Manuscript
