A Systematic Review of the Diverse Applications of Alfalfa and Its Role in Sustainable Agriculture
DOI:
https://doi.org/10.55121/nc.v5i2.1114Abstract
This comprehensive review synthesizes literature from scientific databases, including ScienceDirect, SpringerLink, and Google Scholar, to critically assess the diverse applications of alfalfa (lucerne; Medicago sativa L.). Renowned as a premier forage crop for silage, hay, and grazing, alfalfa delivers exceptional nutritional quality for ruminants, being rich in proteins, vitamins, minerals, and carbohydrates. Beyond its traditional role in animal nutrition, alfalfa is increasingly recognized as a potential functional vegetable for human consumption, with significant medicinal properties, including validated cholesterol-lowering and antidiabetic effects attributed to its bioactive phytochemicals such as saponins and flavonoids. The crop also demonstrates considerable promise for environmental remediation, utilizing its deep root system and high biomass to stabilize heavy metals like cadmium and copper while degrading organic pollutants through phytoremediation mechanisms. Additionally, alfalfa offers viable applications in biofuel and fiber production. Its cultivation is integral to organic and sustainable farming systems, providing critical ecosystem services such as enhancing soil fertility, sequestering carbon, promoting biological nitrogen fixation, and supporting agricultural biodiversity including pollinators, beneficial insects, and avian species of conservation concern. By integrating these nutritional, medicinal, environmental, and agronomic dimensions, this paper consolidates current knowledge to highlight alfalfa’s integral role in promoting resilient and sustainable agricultural systems while addressing interconnected global challenges related to food security, climate change mitigation, and environmental health.
Keywords:
Alfalfa (Medicago sativa), Sustainable Production, Nutritional Quality, Multi-Purpose Crop, Organic Farming, Soil FertilityReferences
[1] Daud, M., Qiao, H., Xu, S., et al., 2025. Understanding Abiotic Stress in Alfalfa: Physiological and Molecular Perspectives on Salinity, Drought, and Heavy Metal Toxicity. Frontiers in Plant Science. 16, 1627599. DOI: https://doi.org/10.3389/fpls.2025.1627599
[2] Lacefield, G., Henning, J., Rasnake, M., et al., 2014. Alfalfa the Queen of Forage Crops, AGR-76. University of Kentucky Cooperative Extension Service: Lexington, KY, USA.
[3] Veronesi, F., Huyghe, C., Delgado, I., 2010. Legumes for Forage and Grain. Food and Agriculture Organization of the United Nations (FAO): Rome, Italy.
[4] Hawkins, C., Yu, L.-X., 2018. Recent Progress in Alfalfa (Medicago sativa L.) Genomics and Genomic Selection. The Crop Journal. 6(6), 565–575. DOI: https://doi.org/10.1016/j.cj.2018.01.006
[5] Wang, S.Y., Yang, G.L., Jing, Y.Y., et al., 2025. Alfalfa quality improvement and loss reduction technology advances. Frontiers in Animal Science. 6, 1550492. DOI: https://doi.org/10.3389/fanim.2025.1550492
[6] Hedayetullah, M., Zaman, P., 2022. Forage Crops of the World, 2-Volume Set: Volume I: Major Forage Crops; Volume II: Minor Forage Crops. CRC Press: Boca Raton, FL, USA.
[7] Baker, K.A., Beecher, L., Northcutt, J.K., 2019. Effect of Irrigation Water Source and Post-Harvest Washing Treatment on the Microflora of Alfalfa and Mung Bean Sprouts. Food Control. 100, 151–157. DOI: https://doi.org/10.1016/j.foodcont.2019.01.015
[8] Mattioli, S., Dal Bosco, A., Castellini, C., et al., 2019. Effect of Heat‐ and Freeze‐Drying Treatments on Phytochemical Content and Fatty Acid Profile of Alfalfa and Flax Sprouts. Journal of the Science of Food and Agriculture. 99, 4029–4035. DOI: https://doi.org/10.1002/jsfa.9630
[9] Michalczyk, M., Fiutak, G., Tarko, T., 2019. Effect of Hot Water Treatment of Seeds on Quality Indicators of Alfalfa Sprouts. LWT. 113, 108270. DOI: https://doi.org/10.1016/j.lwt.2019.108270
[10] Lei, Y., Hannoufa, A., Yu, P., 2017. The Use of Gene Modification and Advanced Molecular Structure Analyses Towards Improving Alfalfa Forage. International Journal of Molecular Sciences. 18(2), 298. DOI: https://doi.org/10.3390/ijms18020298
[11] Julier, B., Gastal, F., Louarn, G., et al., 2017. Lucerne (Alfalfa) in European Cropping Systems. In: Murphy-Bokern, D., Stoddard, F.L., Watson, C.A. (Eds.). Legumes in Cropping Systems. CABI: Wallingford, UK. pp. 168–192. DOI: https://doi.org/10.1079/9781780644981.0168
[12] Gossen, B.D., Ukrainetz, H., Soroka, J.J., 2004. Effect of Fertilizer on Seed Yield of Alfalfa Under Irrigation in Saskatchewan. Canadian Journal of Plant Science. 84(4), 1105–1108. DOI: https://doi.org/10.4141/P03-136
[13] Stanisavljević, R., Beković, D., Djukić, D., et al., 2012. Influence of Plant Density on Yield Components, Yield and Quality of Seed and Forage Yields of Alfalfa Varieties. Romanian Agricultural Research. 29, 245–254.
[14] Dragovoz, I.V., Kots, S.Y., Chekhun, T.I., et al., 2002. Complex Growth Regulator Increases Alfalfa Seed Production. Russian Journal of Plant Physiology. 49, 823–827. DOI: https://doi.org/10.1023/A:1020930016039
[15] Zhang, T., Wang, X., Wang, Y., et al., 2009. Plant Growth Regulator Effects on Balancing Vegetative and Reproductive Phases in Alfalfa Seed Yield. Agronomy Journal. 101(5), 1139–1145. DOI: https://doi.org/10.2134/agronj2009.0017
[16] Haedo, J.P., Martínez, L.C., Graffigna, S., et al., 2022. Managed and wild bees contribute to alfalfa (Medicago sativa) pollination. Agriculture, Ecosystems & Environment. 324, 107711. DOI: https://doi.org/10.1016/j.agee.2021.107711
[17] Zhang, T., Wang, X., Han, J., et al., 2008. Effects of Between‐Row and Within‐Row Spacing on Alfalfa Seed Yields. Crop Science. 48, 794–803. DOI: https://doi.org/10.2135/cropsci2007.06.0340
[18] Peng, W., Cai, W., Pan, J., et al., 2025. Molecular mechanisms of alfalfa response to abiotic stresses. Plants. 14(3), 487. DOI: https://doi.org/10.3390/plants14030487
[19] Sandhu, D., Cornacchione, M.V., Ferreira, J.F., et al., 2017. Variable Salinity Responses of 12 Alfalfa Genotypes and Comparative Expression Analyses of Salt-Response Genes. Scientific Reports. 7, 42958. DOI: https://doi.org/10.1038/srep42958
[20] Stritzler, M., Elba, P., Berini, C., et al., 2018. High-Quality Forage Production under Salinity by Using a Salt-Tolerant AtNXH1-Expressing Transgenic Alfalfa Combined with a Natural Stress-Resistant Nitrogen-Fixing Bacterium. Journal of Biotechnology. 276, 42–45. DOI: https://doi.org/10.1016/j.jbiotec.2018.04.013
[21] Luo, D., Zhou, Q., Wu, Y., et al., 2019. Full-Length Transcript Sequencing and Comparative Transcriptomic Analysis to Evaluate the Contribution of Osmotic and Ionic Stress Components towards Salinity Tolerance in the Roots of Cultivated Alfalfa (Medicago sativa L.). BMC Plant Biology. 19, 32. DOI: https://doi.org/10.1186/s12870-019-1630-4
[22] Liu, Y., Wu, Q., Ge, G., et al., 2018. Influence of Drought Stress on Alfalfa Yields and Nutritional Composition. BMC Plant Biology. 18, 13. DOI: https://doi.org/10.1186/s12870-017-1226-9
[23] Zhao, Y., Wei, X., Ji, X., et al., 2019. Endogenous NO-Mediated Transcripts Involved in Photosynthesis and Carbohydrate Metabolism in Alfalfa (Medicago sativa L.) Seedlings under Drought Stress. Plant Physiology and Biochemistry. 141, 456–465. DOI: https://doi.org/10.1016/j.plaphy.2019.06.023
[24] Zhang, C., Shi, S., Liu, Z., et al., 2019. Drought Tolerance in Alfalfa (Medicago sativa L.) Varieties Is Associated with Enhanced Antioxidative Protection and Declined Lipid Peroxidation. Journal of Plant Physiology. 232, 226–240. DOI: https://doi.org/10.1016/j.jplph.2018.10.023
[25] Kabir, A.H., Hossain, M.M., Khatun, M.A., et al., 2016. Role of Silicon Counteracting Cadmium Toxicity in Alfalfa (Medicago sativa L.). Frontiers in Plant Science. 7, 1117. DOI: https://doi.org/10.3389/fpls.2016.01117
[26] Gu, Y.-J., Han, C.-L., Fan, J.-W., et al., 2018. Alfalfa Forage Yield, Soil Water and P Availability in Response to Plastic Film Mulch and P Fertilization in a Semiarid Environment. Field Crops Research. 215, 94–103. DOI: https://doi.org/10.1016/j.fcr.2017.10.010
[27] Motaharpoor, Z., Taheri, H., Nadian, H., 2019. Rhizophagus irregularis Modulates Cadmium Uptake, Metal Transporter, and Chelator Gene Expression in Medicago sativa. Mycorrhiza. 29, 389–395. DOI: https://doi.org/10.1007/s00572-019-00900-7
[28] Gao, Y.F., Jia, X., Zhao, Y.H., et al., 2023. Glomus mosseae improved the adaptability of alfalfa (Medicago sativa L.) to the coexistence of cadmium-polluted soils and elevated air temperature. Frontiers in Plant Science. 14, 1064732. DOI: https://doi.org/10.3389/fpls.2023.1064732
[29] Samma, M.K., Zhou, H., Cui, W., et al., 2017. Methane Alleviates Copper-Induced Seed Germination Inhibition and Oxidative Stress in Medicago sativa. Biometals. 30, 97–111. DOI: https://doi.org/10.1007/s10534-017-9989-x
[30] Jócsák, I., Knolmajer, B., Szarvas, M., et al., 2022. Literature review on the effects of heavy metal stress and alleviating possibilities through exogenously applied agents in Alfalfa (Medicago sativa L.). Plants. 11(16), 2161. DOI: https://doi.org/10.3390/plants11162161
[31] Duan, C., Razavi, B.S., Shen, G., et al., 2019. Deciphering the Rhizobium Inoculation Effect on Spatial Distribution of Phosphatase Activity in the Rhizosphere of Alfalfa under Copper Stress. Soil Biology and Biochemistry. 137, 107574. DOI: https://doi.org/10.1016/j.soilbio.2019.107574
[32] Ju, W., Liu, L., Fang, L., et al., 2019. Impact of Co-Inoculation with Plant-Growth-Promoting Rhizobacteria and Rhizobium on the Biochemical Responses of Alfalfa-Soil System in Copper Contaminated Soil. Ecotoxicology and Environmental Safety. 167, 218–226. DOI: https://doi.org/10.1016/j.ecoenv.2018.10.016
[33] Raliya, R., Saharan, V., Dimkpa, C., et al., 2017. Nanofertilizer for Precision and Sustainable Agriculture: Current State and Future Perspectives. Journal of Agricultural and Food Chemistry. 66, 6487–6503. DOI: https://doi.org/10.1021/acs.jafc.7b02178
[34] Gopinath, K.A., Visha Kumari, V., Venkatesh, G., et al., 2018. Organic Agriculture: Potentials in Managing Abiotic Stresses in Crop Production. In: Bal, S., Mukherjee, J., Choudhury, B., et al. (Eds.). Advances in Crop Environment Interaction. Springer: Singapore. pp. 229–243. DOI: https://doi.org/10.1007/978-981-13-1861-0_9
[35] Ullah, H., Santiago-Arenas, R., Ferdous, Z., et al., 2019. Improving Water Use Efficiency, Nitrogen Use Efficiency, and Radiation Use Efficiency in Field Crops under Drought Stress: A Review. Advances in Agronomy. 156, 109–157. DOI: https://doi.org/10.1016/bs.agron.2019.02.002
[36] Fahad, S., Bajwa, A.A., Nazir, U., et al., 2017. Crop Production under Drought and Heat Stress: Plant Responses and Management Options. Frontiers in Plant Science. 8, 1147. DOI: https://doi.org/10.3389/fpls.2017.01147
[37] Lamaoui, M., Jemo, M., Datla, R., et al., 2018. Heat and Drought Stresses in Crops and Approaches for Their Mitigation. Frontiers in Chemistry. 6, 26. DOI: https://doi.org/10.3389/fchem.2018.00026
[38] Hussain, H.A., Hussain, S., Khaliq, A., et al., 2018. Chilling and Drought Stresses in Crop Plants: Implications, Cross Talk, and Potential Management Opportunities. Frontiers in Plant Science. 9, 393. DOI: https://doi.org/10.3389/fpls.2018.00393
[39] Sehgal, A., Sita, K., Siddique, K.H., et al., 2018. Drought or/and Heat-Stress Effects on Seed Filling in Food Crops: Impacts on Functional Biochemistry, Seed Yields, and Nutritional Quality. Frontiers in Plant Science. 9, 1705. DOI: https://doi.org/10.3389/fpls.2018.01705
[40] Dahal, K., Li, X.-Q., Tai, H., et al., 2019. Improving Potato Stress Tolerance and Tuber Yield under a Climate Change Scenario—A Current Overview. Frontiers in Plant Science. 10, 563. DOI: https://doi.org/10.3389/fpls.2019.00563
[41] Rodrigues, J., Inzé, D., Nelissen, H., et al., 2019. Source–Sink Regulation in Crops under Water Deficit. Trends in Plant Science. 24(7), 652–663. DOI: https://doi.org/10.1016/j.tplants.2019.04.005
[42] Mielmann, A., 2013. The Utilisation of Lucerne (Medicago sativa): A Review. British Food Journal. 115(4), 590–600. DOI: https://doi.org/10.1108/00070701311317865
[43] Adil, M., 2022. Sustainable Agricultural Practices and Technological Innovations for Climate Change Mitigation in Dryland Farming Systems. Agriculture, Ecosystem and Economics. 1(1). Available from: https://journals.pagepal.org/index.php/AEE/article/view/360
[44] Adil, M., Lv, F., Cao, L., et al., 2024. Long-Term Effects of Agronomic Practices on Winter Wheat Yield and NUE in Dryland Regions of USA and China: A Long-Term Meta-Analysis. Scientific Reports. 14, 24777. DOI: https://doi.org/10.1038/s41598-024-74910-7
[45] Adil, M., Lv, F., Li, T., et al., 2024. Long‐Term Effects of Management Practices on Soil Water, Yield and Water Use of Dryland Wheat: A Global Meta‐Analysis. European Journal of Soil Science. 75(4), e13541. DOI: https://doi.org/10.1111/ejss.13541
[46] Adil, M., Wang, Z., Chen, Y., et al., 2025. Long-Term Impacts of Agronomic Practices on Winter Wheat Yield, Water Use Efficiency, and Nitrogen Use Efficiency in Global Dryland Regions: A Meta-Analysis. Soil and Tillage Research. 253, 106653. DOI: https://doi.org/10.1016/j.still.2025.106653
[47] Adil, M., Zhang, S., Wang, J., et al., 2022. Effects of Fallow Management Practices on Soil Water, Crop Yield and Water Use Efficiency in Winter Wheat Monoculture System: A Meta-Analysis. Frontiers in Plant Science. 13, 825309. DOI: https://doi.org/10.3389/fpls.2022.825309
[48] Ahamad, M.I., Yao, Z., Ren, L., et al., 2024. Impact of Heavy Metals on Aquatic Life and Human Health: A Case Study of River Ravi Pakistan. Frontiers in Marine Science. 11, 1374835. DOI: https://doi.org/10.3389/fmars.2024.1374835
[49] Adil, M., 2023. Precision Remediation of Heavy Metals: Integrating AI, IoT, and Biotechnology for Sustainable Environmental Management. Agriculture, Ecosystem and Economics. 2(1), 1–8. Available from: https://journals.pagepal.org/index.php/AEE/article/view/359
[50] Gul, I., Adil, M., Lu, H., et al., 2025. AI‐Driven Omics for Smart Remediation of Heavy Metal Contaminated Soils. Physiologia Plantarum. 177(6), e70611. DOI: https://doi.org/10.1111/ppl.70611
[51] Gul, I., Adil, M., Lv, F., et al., 2024. Microbial Strategies for Lead Remediation in Agricultural Soils and Wastewater: Mechanisms, Applications, and Future Directions. Frontiers in Microbiology. 15, 1434921. DOI: https://doi.org/10.3389/fmicb.2024.1434921
[52] Aqib, M., Adil, M., Gul, I., 2023. Nanotechnology Applications for Enhancing Irrigation Efficiency and Advancing Sustainability in Precision Agriculture. Agriculture, Ecosystem and Economics. 2(1). Available from: https://journals.pagepal.org/index.php/AEE/article/view/362
[53] Adil, M., Bashir, S., Bashir, S., et al., 2022. Zinc Oxide Nanoparticles Improved Chlorophyll Contents, Physical Parameters, and Wheat Yield under Salt Stress. Frontiers in Plant Science. 13, 932861. DOI: https://doi.org/10.3389/fpls.2022.932861
[54] Iqbal, Y., Ma, W., Li, X., et al., 2025. A Brief Outlook on the Preparation and Magnetic Properties of Iron Oxide Nanoparticles for Water Remediation. Water, Air, & Soil Pollution. 236, 687. DOI: https://doi.org/10.1007/s11270-025-08364-6
[55] Adil, M., Shah, A.N., Khan, A.N., et al., 2023. Amelioration of Harmful Effects of Soil Salinity on Plants through Silicon Application: A Review. Pakistan Journal of Botany. 55(1), 9–18. DOI: https://doi.org/10.30848/PJB2023-1(24)
[56] Adil, M., Yao, Z., Zhang, C., et al., 2022. Climate Change Stress Alleviation through Nature Based Solutions: A Global Perspective. Frontiers in Plant Science. 13, 1007222. DOI: https://doi.org/10.3389/fpls.2022.1007222
[57] Kamran, M., Parveen, A., Ahmar, S., et al., 2019. An Overview of Hazardous Impacts of Soil Salinity in Crops, Tolerance Mechanisms, and Amelioration through Selenium Supplementation. International Journal of Molecular Sciences. 21(1), 148. DOI: https://doi.org/10.3390/ijms21010148
[58] Ali, S., Nadeem, R., Arshad, B., et al., 2022. New Insights in Cropping Patterns, Hydroponic Farming System and Roles in Crop Optimization through Advanced Technologies. Haya: Saudi Journal of Life Sciences. 7(2), 38–43. DOI: https://doi.org/10.36348/sjls.2022.v07i02.003
[59] Javed, M.S., Adil, M., Zhang, J., et al., 2025. Advances in Microbial Bioremediation of Microplastics in Mangrove Sediments: A Comprehensive Review. Water, Air, & Soil Pollution. 236, 927. DOI: https://doi.org/10.1007/s11270-025-08571-1
[60] ul Abadin, Z., Khalid, S., Qamar, S.N., et al., 2021. Risks Factors of Environment Pollutants, Forest and Soil Conservation through Advanced Agriculture Techniques and Future Perspective. Haya: Saudi Journal of Life Sciences. 6(12), 305–309. DOI: https://doi.org/10.36348/sjls.2021.v06i12.002
[61] Raza Altaf, A., Teng, H., Saleem, M., et al., 2021. Associative Interplay of Pseudomonas gessardii BLP141 and Pressmud Ameliorated Growth, Physiology, Yield, and Pb-Toxicity in Sunflower. Bioremediation Journal. 25(2), 178–188. DOI: https://doi.org/10.1080/10889868.2020.1853028
[62] Arshad, M.A., Ansari, N., Umar, M., et al., 2021. A Review on Wheat Management, Strategies, Current Problems and Future Perspectives. Haya: Saudi Journal of Life Sciences. 6(1), 14–18.
[63] Akhter, M., 2021. Novel Aspects of Cotton, Fiber Production in Agriculture and Importance as Staple Crop. Scholars Bulletin. 7(10), 269–272.
[64] Malik, Z., Bashir, M.A., Abbasi, G.H., et al., 2021. Impact of Environmental and Edaphic Factors on Winter Fodders and Remedies. In: ul Haq, I., Ijaz, S. (Eds.). Sustainable Winter Fodder. CRC Press: Boca Raton, FL, USA. pp. 223–253. DOI: https://doi.org/10.1201/9781003055365-11
[65] Hussain, A., Ahmad, M., Zia, Z., et al., 2021. Methods and Biological Factors Affecting for Plant Microbes and Stomatal Interaction, Seed Hybrid Technology through Genetic Engineering. Saudi Journal of Pathology and Microbiology. 6(11), 406–410.
[66] Parveen, Z., Rafique, R.S., Mushtaq, M., et al., 2021. New Insights in Genetically Modified Crops and Plant Diseases through Molecular Biology. Haya: Saudi Journal of Life Sciences. 6(11), 279–283.
[67] Sen, S., Makkar, H.P., Becker, K., 1998. Alfalfa Saponins and Their Implication in Animal Nutrition. Journal of Agricultural and Food Chemistry. 46, 131–140. DOI: https://doi.org/10.1021/jf970389i
[68] Odhav, B., Beekrum, S., Akula, U.S., et al., 2007. Preliminary Assessment of Nutritional Value of Traditional Leafy Vegetables in KwaZulu-Natal, South Africa. Journal of Food Composition and Analysis. 20(5), 430–435. DOI: https://doi.org/10.1016/j.jfca.2006.04.015
[69] Mölgaard, J., von Schenck, H., Olsson, A.G., 1987. Alfalfa Seeds Lower Low-Density Lipoprotein Cholesterol and Apolipoprotein B Concentrations in Patients with Type II Hyperlipoproteinemia. Atherosclerosis. 65(1), 173–179. DOI: https://doi.org/10.1016/0021-9150(87)90019-0
[70] Gray, A.M., Flatt, P.R., 1997. Pancreatic and Extra-Pancreatic Effects of the Traditional Anti-Diabetic Plant, Medicago sativa (Lucerne). British Journal of Nutrition. 78(2), 325–334. DOI: https://doi.org/10.1079/BJN19970150
[71] Suwignyo, B., Kurniawan, F.D., Suseno, N., et al., 2020. Productivity and Nutrient Content of the Second Regrowth Alfalfa (Medicago sativa L.) with Different Photoperiod and Dolomite. Animal Production. 22(2), 74–81.
[72] Suwignyo, B., Mustika, A., Kustantinah, L.M.Y., et al., 2020. Effect of Drying Method on Physical-Chemical Characteristics and Amino Acid Content of Tropical Alfalfa (Medicago sativa L.) Hay for Poultry Feed. American Journal of Animal and Veterinary Sciences. 15(2), 118–122. DOI: https://doi.org/10.3844/ajavsp.2020.118.122
[73] Suwignyo, B., Suryanto, E., Sasongko, H., et al., 2020. The Effect of Fresh and Hay Alfalfa (Medicago sativa L.) Supplementation on Carcass Quality of Hybrid Duck. IOP Conference Series: Earth and Environmental Science. 478, 012024. DOI: https://doi.org/10.1088/1755-1315/478/1/012024
[74] Suwignyo, B., Rini, E.A., Fadli, M.K., et al., 2021. Effects of Alfalfa (Medicago sativa L.) Supplementation in the Diet on the Growth, Small Intestinal Histomorphology, and Digestibility of Hybrid Ducks. Veterinaria World. 14(10), 2719–2726. DOI: https://doi.org/10.14202/vetworld.2021.2719-2726
[75] Samur, S.I.N., Suwignyo, B., Suryanto, E., 2020. The Effect of Alfalfa (Medicago sativa L.) on Different Basal Feeds for Hybrid Duck Performance. E3S Web of Conferences. 200, 03013. DOI: https://doi.org/10.1051/e3sconf/202020003013
[76] Sulaiman, B.F., Al-Sardary, S.Y.T., 2021. Alfalfa Meal Supplementation Producing Vitamin E and Minerals Enriched Table Eggs. IOP Conference Series: Earth and Environmental Science. 761, 012108. DOI: https://doi.org/10.1088/1755-1315/761/1/012108
[77] Hao, C.C., Wang, L.J., Li, D., et al., 2008. Influence of Alfalfa Powder Concentration and Granularity on Rheological Properties of Alfalfa-Wheat Dough. Journal of Food Engineering. 89(2), 137–141. DOI: https://doi.org/10.1016/j.jfoodeng.2008.04.011
[78] Oleszek, W.A., 2002. Chromatographic Determination of Plant Saponins. Journal of Chromatography A. 967(1), 147–162. DOI: https://doi.org/10.1016/S0021-9673(01)01556-4
[79] Griffiths, F.P., 1949. Production and Utilization of Alfalfa. Economic Botany. 3, 170–183. DOI: https://doi.org/10.1007/BF02859523
[80] Suwignyo, B., Izzati, F., Astuti, A., et al., 2020. Nutrient Content of Alfalfa (Medicago sativa L.) Regrowth I in Different Fertilizers and Lighting. IOP Conference Series: Earth and Environmental Science. 465, 012035. DOI: https://doi.org/10.1088/1755-1315/465/1/012035
[81] Murod, M., Suwignyo, B., Ariyadi, B., 2025. The effect of tropical alfalfa (Medicago sativa L. cv Kacang Ratu BW) supplementation on performance, intestinal histomorphology, and nutrient digestibility in hybrid ducks. Veterinary Integrative Sciences. 23(1), 1–14. DOI: https://doi.org/10.12982/VIS.2025.008
[82] Suwignyo, B., Suryanto, E., Samur, S.I.N., et al., 2021. The Effect of Hay Alfalfa (Medicago sativa L.) Supplementation in Different Basal Feed on the Feed Intake (FI), Body Weight, and Feed Conversion Ratio of Hybrid Ducks. IOP Conference Series: Earth and Environmental Science. 686, 012039. DOI: https://doi.org/10.1088/1755-1315/686/1/012039
[83] Bolton, J.L., 1962. Alfalfa: Botany, Cultivation and Utilization. Leonard Hill: London, UK.
[84] Seven, P.T., Seven, I., Mutlu, S.I., et al., 2021. Silage additives usage in improving fermentation quality of alfalfa silage: A review. Animal and Veterinary Sciences. 9(6), 175–180. DOI: https://doi.org/10.11648/j.avs.20210906.13
[85] Levy, L.F., Fox, F.W., 1935. Antiscorbutic Value of Lucerne. Biochemical Journal. 29(4), 884–888. DOI: https://doi.org/10.1042/bj0290884
[86] Peñas, E., Gómez, R., Frías, J., et al., 2009. Efficacy of Combinations of High-Pressure Treatment, Temperature and Antimicrobial Compounds to Improve the Microbiological Quality of Alfalfa Seeds for Sprout Production. Food Control. 20(1), 31–39. DOI: https://doi.org/10.1016/j.foodcont.2008.01.012
[87] Kalač, P., Price, K.R., Fenwick, G.R., 1996. Changes in Saponin Content and Composition during the Ensilage of Alfalfa (Medicago sativa L.). Food Chemistry. 56(4), 377–380. DOI: https://doi.org/10.1016/0308-8146(95)00185-9
[88] Karolkowski, A., Belloir, C., Briand, L., et al., 2023. Non-volatile compounds involved in bitterness and astringency of pulses: A review. Molecules. 28(8), 3298. DOI: https://doi.org/10.3390/molecules28083298
[89] Tilla, I., Blumberga, D., 2018. Qualitative Indicator Analysis of a Sustainable Remediation. Energy Procedia. 147, 588–593. DOI: https://doi.org/10.1016/j.egypro.2018.07.075
[90] Wu, H., Lai, C., Zeng, G., et al., 2017. The Interactions of Composting and Biochar and Their Implications for Soil Amendment and Pollution Remediation: A Review. Critical Reviews in Biotechnology. 37(6), 754–764. DOI: https://doi.org/10.1080/07388551.2016.1232696
[91] Ye, J., Chen, X., Chen, C., et al., 2019. Emerging Sustainable Technologies for Remediation of Soils and Groundwater in a Municipal Solid Waste Landfill Site—A Review. Chemosphere. 227, 681–702. DOI: https://doi.org/10.1016/j.chemosphere.2019.04.053
[92] Emenike, C.U., Agamuthu, P., Fauziah, S.H., 2017. Sustainable Remediation of Heavy Metal Polluted Soil: A Biotechnical Interaction with Selected Bacteria Species. Journal of Geochemical Exploration. 182, 275–278. DOI: https://doi.org/10.1016/j.gexplo.2016.10.002
[93] Rostami, S., Azhdarpoor, A., 2019. The Application of Plant Growth Regulators to Improve Phytoremediation of Contaminated Soils: A Review. Chemosphere. 220, 818–827. DOI: https://doi.org/10.1016/j.chemosphere.2018.12.203
[94] Agnello, A.C., Huguenot, D., van Hullebusch, E.D., et al., 2016. Citric Acid- and Tween® 80-Assisted Phytoremediation of a Co-Contaminated Soil: Alfalfa (Medicago sativa L.) Performance and Remediation Potential. Environmental Science and Pollution Research. 23, 9215–9226. DOI: https://doi.org/10.1007/s11356-015-5972-7
[95] Chen, F., Dong, W., Zhang, J., et al., 2018. The Sequenced Angiosperm Genomes and Genome Databases. Frontiers in Plant Science. 9, 418. DOI: https://doi.org/10.3389/fpls.2018.00418
[96] Yang, S., Zu, Y., Li, B., et al., 2019. Response and Intraspecific Differences in Nitrogen Metabolism of Alfalfa (Medicago sativa L.) under Cadmium Stress. Chemosphere. 220, 69–76. DOI: https://doi.org/10.1016/j.chemosphere.2018.12.101
[97] Teng, Y., Sun, X., Zhu, L., et al., 2017. Polychlorinated Biphenyls in Alfalfa: Accumulation, Sorption and Speciation in Different Plant Parts. International Journal of Phytoremediation. 19(8), 732–738. DOI: https://doi.org/10.1080/15226514.2017.1284749
[98] Tu, C., Ma, L., Guo, P., et al., 2017. Rhizoremediation of a Dioxin-Like PCB Polluted Soil by Alfalfa: Dynamic Characterization at Temporal and Spatial Scale. Chemosphere. 189, 517–524. DOI: https://doi.org/10.1016/j.chemosphere.2017.09.091
[99] Alves, W.S., Manoel, E.A., Santos, N.S., et al., 2017. Detection of Polycyclic Aromatic Hydrocarbons (PAHs) in Medicago sativa L. by Fluorescence Microscopy. Micron. 95, 23–30. DOI: https://doi.org/10.1016/j.micron.2017.01.004
[100] Alves, W.S., Manoel, E.A., Santos, N.S., et al., 2018. Phytoremediation of Polycyclic Aromatic Hydrocarbons (PAH) by cv. Crioula: A Brazilian Alfalfa Cultivar. International Journal of Phytoremediation. 20(8), 747–755. DOI: https://doi.org/10.1080/15226514.2018.1425663
[101] Russelle, M.P., Lamb, J.F.S., Turyk, N.B., et al., 2007. Managing Nitrogen Contaminated Soils: Benefits of N₂-Fixing Alfalfa. Agronomy Journal. 99(3), 738–746. DOI: https://doi.org/10.2134/agronj2005.0325
[102] Blumenthal, J.M., Russelle, M.P., 1996. Subsoil Nitrate Uptake and Symbiotic Dinitrogen Fixation by Alfalfa. Agronomy Journal. 88(6), 909–915. DOI: https://doi.org/10.2134/agronj1996.00021962003600060010x
[103] Rochette, P., Angers, D.A., Bélanger, G., et al., 2004. Emissions of N₂O from Alfalfa and Soybean Crops in Eastern Canada. Soil Science Society of America Journal. 68(2), 493–506. DOI: https://doi.org/10.2136/sssaj2004.4930
[104] Wagner-Riddle, C., Thurtell, G.W., 1998. Nitrous Oxide Emissions from Agricultural Fields during Winter and Spring Thaw as Affected by Management Practices. Nutrient Cycling in Agroecosystems. 52, 151–163. DOI: https://doi.org/10.1023/A:1009788411566
[105] Mortenson, M.C., Schuman, G.E., Ingram, L.J., 2004. Carbon Sequestration in Rangelands Interseeded with Yellow-Flowering Alfalfa (Medicago sativa ssp. falcata). Environmental Management. 33(Suppl 1), S475–S481. DOI: https://doi.org/10.1007/s00267-003-9155-9
[106] Kelner, D.J., Vessey, J.K., Entz, M.H., 1997. The Nitrogen Dynamics of 1-, 2- and 3-Year Stands of Alfalfa in a Cropping System. Agriculture, Ecosystems & Environment. 64(1), 1–10. DOI: https://doi.org/10.1016/S0167-8809(97)00019-4
[107] Angus, J.F., Peoples, M.B., 2012. Nitrogen from Australian Dryland Pastures. Crop & Pasture Science. 63, 746–758. DOI: https://doi.org/10.1071/CP12161
[108] Rasse, D.P., Smucker, A.J., Schabenberger, O., 1999. Modifications of Soil Nitrogen Pools in Response to Alfalfa Root Systems and Shoot Mulch. Agronomy Journal. 91(3), 471–477. DOI: https://doi.org/10.2134/agronj1999.00021962009100030019x
[109] Justes, E., Thiebeau, P., Cattin, G., et al., 2001. Nitrogen Release after Plowing under Alfalfa: An Effect Over Two Growing Seasons. Perspectives Agricoles. 264, 22–28. (in French)
[110] Baldock, J.O., Higgs, R.L., Paulson, W.H., et al., 1981. Legume and Mineral N Effects on Crop Yields in Several Crop Sequences in the Upper Mississippi Valley. Agronomy Journal. 73(5), 885–890. DOI: https://doi.org/10.2134/agronj1981.00021962007300050031x
[111] Bruulsema, T.W., Christie, B.R., 1987. Nitrogen Contribution to Succeeding Corn from Alfalfa and Red Clover. Agronomy Journal. 79(1), 96–100. DOI: https://doi.org/10.2134/agronj1987.00021962007900010020x
[112] Hesterman, O.B., Russelle, M.P., Sheaffer, C.C., et al., 1987. Nitrogen Utilization from Fertilizer and Legume Residues in Legume‐Corn Rotations. Agronomy Journal. 79(4), 726–731. DOI: https://doi.org/10.2134/agronj1987.00021962007900040029x
[113] Ballesta, A., Lloveras, J., 2010. Nitrogen Replacement Value of Alfalfa to Corn and Wheat Under Irrigated Mediterranean Conditions. Spanish Journal of Agricultural Research. 8(1), 159–169. DOI: https://doi.org/10.5424/sjar/2010081-1155
[114] Cela, S., Santiveri, F., Lloveras, J., 2011. Optimum Nitrogen Fertilization Rates for Second-Year Corn Succeeding Alfalfa under Irrigation. Field Crops Research. 123(2), 109–116. DOI: https://doi.org/10.1016/j.fcr.2011.05.003
[115] Vertès, F., Jeuffroy, M.-H., Louarn, G., et al., 2015. Legume Use in Temporary Pastures: Supplying Nitrogen in Crop-Rotation Systems. Fourrages. 223, 221–232. (in French)
[116] Brophy, L.S., Heichel, G.H., 1989. Nitrogen Release from Roots of Alfalfa and Soybean Grown in Sand Culture. Plant and Soil. 116, 77–84. DOI: https://doi.org/10.1007/BF02327259
[117] Lory, J.A., Russelle, M.P., Heichel, G.H., 1992. Quantification of Symbiotically Fixed Nitrogen in Soil Surrounding Alfalfa Roots and Nodules. Agronomy Journal. 84(6), 1033–1040. DOI: https://doi.org/10.2134/agronj1992.00021962008400060024x
[118] Dubach, M., Russelle, M.P., 1994. Forage Legume Roots and Nodules and Their Role in Nitrogen Transfer. Agronomy Journal. 86(2), 259–266. DOI: https://doi.org/10.2134/agronj1994.00021962008600020010x
[119] Louarn, G., Pereira-Lopès, E., Fustec, J., et al., 2015. The Amounts and Dynamics of Nitrogen Transfer to Grasses Differ in Alfalfa and White Clover-Based Grass-Legume Mixtures as a Result of Rooting Strategies and Rhizodeposit Quality. Plant and Soil. 389, 289–305. DOI: https://doi.org/10.1007/s11104-014-2354-8
[120] Angus, J.F., Gault, R.R., Good, A.J., et al., 2000. Lucerne Removal before a Cropping Phase. Australian Journal of Agricultural Research. 51, 877–889. DOI: https://doi.org/10.1071/AR99183
[121] Angus, J.F., Bolger, T.P., Kirkegaard, J.A., et al., 2006. Nitrogen Mineralisation in Relation to Previous Crops and Pastures. Australian Journal of Soil Research. 44, 355–365. DOI: https://doi.org/10.1071/SR05138
[122] Rollin, O., Bretagnolle, V., Decourtye, A., et al., 2013. Differences of Floral Resource Use between Honeybees and Wild Bees in an Intensive Farming System. Agriculture, Ecosystems & Environment. 179, 78–86. DOI: https://doi.org/10.1016/j.agee.2013.07.007
[123] Thiebeau, P., Badenhausser, I., Meiss, H., et al., 2010. Contribution of Legumes to the Biodiversity of Rural Landscapes. Innovations Agronomiques. 11, 187–204. DOI: https://doi.org/10.17180/2m6w-jh64 (in French)
[124] Kragten, S., Trimbos, K.B., de Snoo, G.R., 2008. Breeding Skylarks (Alauda arvensis) on Organic and Conventional Arable Farms in the Netherlands. Agriculture, Ecosystems & Environment. 126(3–4), 163–167. DOI: https://doi.org/10.1016/j.agee.2008.01.021
[125] Salamolard, M., Butet, A., Leroux, A., et al., 2000. Responses of an Avian Predator to Variations in Prey Density at a Temperate Latitude. Ecology. 81(9), 2428–2441. DOI: https://doi.org/10.1890/0012-9658(2000)081[2428:ROAAPT]2.0.CO;2
[126] Berthet, E.T., Bretagnolle, V., Segrestin, B., 2012. Analyzing the Design Process of Farming Practices Ensuring Little Bustard Conservation: Lessons for Collective Landscape Management. Journal of Sustainable Agriculture. 36(3), 319–336. DOI: https://doi.org/10.1080/10440046.2011.627988
[127] Raynal, G., Courtillot, M., Bournoville, R., et al., 1989. Enemies and Diseases of Meadows/Pastures. Institut National de la Recherche Agronomique (INRA): Paris, France. pp. 1–252. (in French)
[128] Badenhausser, I., Gouat, M., Goarant, A., et al., 2012. Spatial Autocorrelation in Farmland Grasshopper Assemblages (Orthoptera: Acrididae) in Western France. Environmental Entomology. 41(5), 1050–1061. DOI: https://doi.org/10.1603/EN11256
[129] Inchausti, P., Carslake, D., Attié, C., et al., 2009. Is There Direct and Delayed Density Dependent Variation in Population Structure in a Temperate European Cyclic Vole Population? Oikos. 118(8), 1201–1211. DOI: https://doi.org/10.1111/j.1600-0706.2009.17488.x
[130] Razzaq, S., Zhou, B., Adil, M., et al., 2024. Cadmium Stress Alleviation: Interplay of Micronutrients and Enzymatic/Non-enzymatic Species in Maize by Organic and Inorganic Amendments. Water, Air, & Soil Pollution. 235, 305. DOI: https://doi.org/10.1007/s11270-024-07086-5
[131] Razzaq, S., Zhou, B., Ullah, Z., et al., 2024. Exploring the Impact of Organic and Inorganic Amendments, with Foliar Application of Iron Nanoparticles, on Cadmium Stabilization and Growth of Maize in Wastewater Irrigated-Soil. Journal of Hazardous Materials Letters. 5, 100111. DOI: https://doi.org/10.1016/j.hazl.2024.100111
[132] Bashir, H., Almutlaq, N., Ghafoor, A., et al., 2025. Divergent Roles of Vermicompost and Its Derivative Biochar in Cadmium Immobilization, Soil Health, and Rice Productivity. Authorea. in Press. DOI: https://doi.org/10.22541/au.175952340.05418629/v1
[133] Raheel, M., 2021. Different Methods for Detection of Nanoparticles for Semiconductors and Photovoltaic Cells through Diffraction & Novel Approaches. Scholars Bulletin. 7(10), 264–268.
[134] Iqbal, Y., Wang, C., Hussain, S., et al., 2025. N-Type Mg₃Sb₂ (Bi, Te) Alloy Preparation and Its Thermoelectric Performance Enhancement. Journal of Materials Engineering and Performance. 35, 2466–2475. DOI: https://doi.org/10.1007/s11665-025-11753-x
[135] Ikram Ullah, M.A.A.G., Nadeem, S., Mahmood, A., et al., 2021. Effects of Different Doses of Humic Acid and Sowing Methods on Growth and Yield of Turnip (Brassica rapa L.). Bioscience Research. 18(4), 3065–3073.
[136] ul Abadin, Z., 2021. Agricultural Industrial Significance of Cotton, Types, Cotton Varieties and Role in Disease Control. Scholars Bulletin. 7(8), 225–228.
[137] Adil, M., Gul, I., Lu, S., et al., 2026. Nanotechnology for Climate-Resilient Agriculture: Advancing Water Efficiency, Crop Resilience, and Sustainable Food Security. Water, Air, & Soil Pollution. 237(11), 653.
[138] Saleem, S., Hassan, W., Adil, M., et al., 2024. Sustainable Management of Saline Soils by Using Organic and Inorganic Amendments: Implications for Wheat Growth and Production. Plant and Environment. 5(1), 100–111.
[139] Yu, T., Deng, Y., Muhammad, A., et al., 2026. Long Term Dynamics and Drivers of Bioavailable Silicon from Source to Sink in Taihu Watershed, China. Journal of Environmental Sciences. in Press. DOI: https://doi.org/10.1016/j.jes.2026.03.016
[140] Adil, M., Lu, S., Yao, Z., et al., 2024. No-Tillage Enhances Soil Water Storage, Grain Yield and Water Use Efficiency in Dryland Wheat (Triticum aestivum) and Maize (Zea mays) Cropping Systems: A Global Meta-Analysis. Functional Plant Biology. 51(5), FP23267. DOI: https://doi.org/10.1071/FP23267
[141] Adil, M., Zhang, C., Yao, Z., et al., 2023. Interactive Effects of Intercropping and Mulching under Conservation Tillage as a Sustainable Agriculture Increased Cotton Productivity. Frontiers in Ecology and Evolution. 10, 1092636. DOI: https://doi.org/10.3389/fevo.2022.1092636
Downloads
How to Cite
Issue
Section
License
Copyright (c) 2026 Muhammad Adil, Usama Shoukat, Muhammad Daud, Isma Gul, Rifqa Afzal, Muhammad Aqib, Sajjad Shafeeq

This work is licensed under a Creative Commons Attribution 4.0 International License.
Submit Manuscript
