Evaluation of Morphological Variations in Exotic Rice (Oryza Sativa L.) Genetic Materials under Sri Lankan Field Conditions


  • Deepika Priyadarshani Weerasinghe Department of Agriculture




Rice, Germplasm, Cluster analysis, Genetic diversity


To evaluate genetic diversity of 64 rice germplasms with five local check varieties, an experiment was conducted as the randomized complete block design with two replicates. It was conducted in experimental field of the Regional Rice Research and Development Center, Bombuwela, Sri Lanka. There were five morphological characters such as plant height, days to flowering, days to maturity, grain yield and the phenotypic acceptability of varieties were evaluated. As the results analysis of variance showed there is a significant difference among genotypes of all the tested characters. A total of four clusters were defined through cluster analysis and the distinct genetic variations were observed among the clusters. Cluster i consisted of six genotypes, cluster ii of 38, cluster iii of 19 and in cluster iv of one genotype were separated. Cluster groupings of local rice varieties into three main groups from three different rice breeding stations such as Bombuwela (Bw), Ambalantota (At) and Batalagoda (Bg) resemble their distance in genetic diversity as well as close genetic parental materials used in developing rice varieties within a cluster. Subsequent analysis of five tested variables confirms significant (p < 0.01) positive correlation (r = 0.61) between plant height and yield among the tested rice varieties. Depending on the breeding objectives the results of multivariate analysis can be applied for the rice varietal improvement program


[1] IRRI, 2014. Standard Evaluation System for Rice. INGER, genetic resources center. International rice research institute, 1099, Manila, Philippines.

[2] Wang, S., Wu, K., Yuan, Q., et al., 2012. Control of grain size, shape and quality by OsSPL16 in rice. Nature Genetics. 44, 950-954.

[3] DOI: https://doi.org/10.1038/ng.2327

[4] Zeng, D., Tian, Z., Rao, Y., et al., 2017. Rational design of high-yield and superior-quality rice. Nature Plants. 3, 17031.

[5] DOI: https://doi.org/10.1038/nplants.2017.31

[6] Zhang, L., Yu, H., Ma, B., et al., 2017. A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice. Nature Communications. 8, 14789.

[7] DOI: https://doi.org/10.1038/ncomms14789

[8] Slafer, G.A., 1994. Genetic Improvements of Field Crops. pp. 68. Marcel Dekker Inc., New York, USA.

[9] Richards, R.A., 2000. Selectable traits to increase crop 17. Photosynthesis and yield of grain crops. Journal of Experimental Botany. 51(1), 447-458.

[10] Moldenhauer, K., Nathan, S., 2004. 1-Rice growth and development. Slaton, N. (Ed.), Rice Production Handbook. Arkansas: University of Arkansas.

[11] Sakamoto, T., Matsuoka, M., 2008. Identifying and exploiting grain yield genes in rice. Current Opinion in Plant Biology. 11, 209-214.

[12] DOI: https://doi.org/10.1016/j.pbi.2008.01.009

[13] Huang, R., Jiang, L., Zheng, J., et al., 2013. Genetic bases of rice grain shape: so many genes, so little known. Trends in Plant Science. 18, 218-226.

[14] DOI: https://doi.org/10.1016/j.tplants.2012.11.001

[15] Yano, M., Kojima, S., Takahashi, Y., et al., 2001. Genetic control of flowering time in rice, a short-day plant. Plant Physiology. 127, 1425-1429.

[16] Xue, D., Qian, Q., Teng, S., 2014. Identification and Utilization of Elite Genes from Elite Germplasms for Yield Improvement. (Retrieved on 13.05.2014) http://cdn.intechopen.com/pdfs-wm/45539.pdf.

[17] DOI: http://dx.doi.org/10.5772/56390

[18] Yano, M.Y., Harushima, Y., Nagamura, N., et al., 1997. Identification of quantitative trait loci controlling heading date of rice using a high-density linkage map. Theoretical and Applied Genetics. 95, 1025-1032.

[19] Agstat, 2018. Agricultural statistics, Socio Economics and Planning Center, Department of Agriculture, Peradaeniya, Sri Lanka. pp. 18.

[20] Spielmeyer, W., Ellis, M.H., Chandler, P.M., 2002. Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proceedings of the National Academy of Sciences of the United States of America. 99, 9043-9048.

[21] DOI: https://doi.org/10.1073/pnas.132266399

[22] Kovi, M.R., Zhang, Y., Yu, S., et al., 2011. Candidacy of a chitin-inducible gibberellin-responsive gene for a major locus affecting plant height in rice that is closely linked to green revolution gene sd1. Theoretical and Applied Genetics.123, 705-714.

[23] DOI: https://doi.org/10.1007/s00122-011-1620-x

[24] Zhang, Y., Yu, C., Lin, J., et al., 2017. OsMPH1 regulates plant height and improves grain yield in rice. PLoS One. 12, e0180825.

[25] DOI: https://doi.org/10.1371/journal.pone.0180825




How to Cite

Weerasinghe, D. P. (2022). Evaluation of Morphological Variations in Exotic Rice (Oryza Sativa L.) Genetic Materials under Sri Lankan Field Conditions. New Countryside, 1(2), 16–22. https://doi.org/10.55121/nc.v1i2.32